Transposition of Tn917 in Bacillus megaterium
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چکیده
منابع مشابه
Transposition of Tn917 in Bacillus megateriumt
Transposon Tn9l7 from Steptococcus faecalis (12) is presently the most useful transposon for Bacillus subtilis genetic manipulation. It is 5.3 kilobases in length, confers inducible erythromycin resistance, and has terminal inverted repeats very closely related to the Tn3 family of transposons of gram-negative organisms (11). Youngman et al. (16) constructed a Tn917-carrying plasmid, pTV1, that...
متن کاملChromosomal insertions of Tn917 in Bacillus subtilis.
We describe 46 insertions of the Streptococcus faecalis transposon Tn917 into the chromosome of Bacillus subtilis. These insertion mutations were mapped genetically. Some caused auxotrophic requirements, and others were cryptic. These insertions were scattered around the B. subtilis chromosome. The mutant strains were useful in several ways for mapping and cloning B. subtilis genes and were add...
متن کاملPlasmolysis in Bacillus Megaterium.
Weibull, Claes (Central Bacteriological Laboratory of Stockholm City, Stockholm, Sweden). Plasmolysis in Bacillus megaterium. J. Bacteriol. 89:1151-1154. 1965.-Sucrose solutions stronger than 1 m caused plasmolysis in Bacillus megaterium strain M, whereas concentrated NaCl and KNO(3) solutions were ineffective. In plasmolyzed cells, mesosome bodies were found in pockets between the cytoplasmic ...
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The streptococcal transposon Tn917 was demonstrated to transpose in Escherichia coli from the Bacillus subtilis-E. coli shuttle plasmid pHK1207 into an F' plasmid derivative. Subsequently, a second round of transposition from the F' plasmid into pACYC184 could be readily demonstrated. These results represent the initial demonstration of the transposition of a gram-positive transposon in a gram-...
متن کاملEnzyme localization in Bacillus megaterium.
Weibull (1953a) has shown that after osmotic lysis of protoplasts of Bacillus megaterium, prepared by controlled treatment with lysozyme, only two microscopically detectable structures remain; membrane-like bodies termed "ghosts," and granules. The ghosts were found to sediment as a dark yellow fraction by centrifugation at 10,000 G (Weibull, 1953b) and to contain almost all the pigmented mater...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1986
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.167.2.716-718.1986